Protein Engineering: Tools and Applications

Huimin Zhao · Sang Yup Lee · Jens Nielsen · Gregory Stephanopoulos

gush 2021 · John Wiley & Sons

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A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology

Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications.

The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book:

Covers technologies and methodologies used in protein engineering
Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications
Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology
Presents cutting edge advances in the continuously evolving field of protein engineering

Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

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Dr. Huimin Zhao is the Steven L. Miller Chair of chemical and biomolecular engineering, and professor of chemistry, biochemistry, biophysics, and bioengineering at the University of Illinois at Urbana-Champaign (UIUC). He received his B.S. degree in Biology from the University of Science and Technology of China in 1992 and his Ph.D. degree in Chemistry from the California Institute of Technology in 1998 under the guidance of Dr. Frances Arnold. Prior to joining UIUC in 2000, he was a project leader at the Industrial Biotechnology Laboratory of the Dow Chemical Company. He was promoted to full professor in 2008. Dr. Zhao served as a consultant for over 10 companies such as Pfizer, Maxygen, BP, Gevo, and zuChem, and a Scientific Advisory Board member of Gevo, Myriant Technologies, Toulouse White Biotechnology (TWB) and AgriMetis. He was a member of National Academies' study group on Industrialization of Biology: A Roadmap to Accelerate Advanced Manufacturing of Chemicals. Dr. Zhao has authored and co-authored over 260 research articles and over 20 issued and pending patent applications with several being licensed by industry. In addition, he has given plenary, keynote or invited lectures in over 290 international meetings, universities, industries, and research institutes. His primary research interests are in the development and applications of synthetic biology tools to address society's most daunting challenges in health, energy, and sustainability, and in the fundamental aspects of enzyme catalysis, cell metabolism, and gene regulation.

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