This volume constitutes the thoroughly refereed post-proceedings of the 7th International Workshop on Agent-Oriented Software Engineering, AOSE 2006, held in Hakodate, Japan, in May 2006 as part of AAMAS 2006. The 13 revised full papers were carefully selected from numerous submissions during two rounds of reviewing and improvement and have been complemented by invited papers from leading researchers in the field. The papers are organized in topical sections on modelling and design of agent systems, modelling open agent systems, formal reasoning about designs, as well as testing, debugging and evolvability.