Circadian Rhythms: Methods and Protocols

Ezio Rosato

2008년 2월 · Methods in molecular biology 362권 · Springer Science & Business Media

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Circadian Rhythms: Methods and Protocols is an in-depth examination of
circadian biology, presented by leading researchers in the field. This
cutting-edge collection of protocols is adaptable for research at every
level, and represents the huge strides that chronobiologists have made
over the past two decades. These robust, reproducible procedures take
into account the myriad of variables that circadian researchers must be
cognizant of while conducting their research. By distilling the
arsenal of methodologies available, and presenting essential procedures
in precise detail, Circadian Rhythms: Methods and Protocols supports a
vast majority of circadian research efforts.

Part I of this seven-part volume provides a concise introduction to
essential components of circadian biology, including light,
photoreceptors, circadian clocks, and statistical analysis. Part II
presents protocols and techniques for monitoring and analyzing rhythmic
patterns of several model organisms. Parts III, IV, and V emphasize
the identification of clock genes and protein and RNA expression in
circadian biology. Protocols for in vitro systems comprise Part VI,
and Part VII provides an outline of methods and tips for microscopy
analysis. Circadian biologists at all research levels will realize
tremendous benefit from this extraordinary collection of methods and
protocols for the study of circadian rhythms.