Capillary Electrophoresis of Carbohydrates
Pierre Thibault · Susumu Honda
2/2008 · Methods in molecular biology 213. kniha · Springer Science & Business Media
E‑kniha
318
Počet strán
reportHodnotenia a recenzie nie sú overené Ďalšie informácie
Táto e‑kniha
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
Ohodnoťte túto elektronickú knihu
Povedzte nám svoj názor.
Informácie o dostupnosti
Smartfóny a tablety
Nainštalujte si aplikáciu Knihy Google Play pre Android a iPad/iPhone. Automaticky sa synchronizuje s vaším účtom a umožňuje čítať online aj offline, nech už ste kdekoľvek.
Laptopy a počítače
Audioknihy zakúpené v službe Google Play môžete počúvať prostredníctvom webového prehliadača v počítači.
Čítačky elektronických kníh a ďalšie zariadenia
Ak chcete tento obsah čítať v zariadeniach využívajúcich elektronický atrament, ako sú čítačky e‑kníh Kobo, musíte stiahnuť príslušný súbor a preniesť ho do svojho zariadenia. Pri prenose súborov do podporovaných čítačiek e‑kníh postupujte podľa podrobných pokynov v centre pomoci.
